2017-12-15 17:12:032025-05-13 17:40:31
locus
BSU39080
BSU_39080
geneLength
831
834
outlinks
bsu
BSU39080
BSU_39080
Gene
Phenotypes of a mutant
no expression of the ''[[gene|bglP]]-[[gene|bglH]]'' operon
no expression of the [[gene|bglP]]-[[gene|bglH]] operon
The protein
Protein family
BglG family of antiterminators (according to Swiss-Prot)
[SW|PRD-containing transcription factors]
The protein
Paralogous protein(s)
[[protein|SacY]], [[protein|GlcT]], [[protein|SacT]]
[[this]]
The protein
[SW|Domains]
N-terminal RNA binding domain [Pubmed|10610766]
2 x [SW|PRD] ([SW|PTS] regulation domains) [Pubmed|11447120]
N-terminal RNA binding domain [Pubmed|10610766]
2 x [SW|PRD] ([SW|PTS] regulation domains) [Pubmed|11447120]
2 [SW|PRD] domains (aa 65-170, aa 171-277) (according to UniProt)
Biological materials
Expression vector
for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP165, available in [SW|Jörg Stülke]'s lab
for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP315, available in [SW|Jörg Stülke]'s lab
for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP572, available in [SW|Jörg Stülke]'s lab
Biological materials
GFP fusion
GP1225 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab
GP1225, [[gene|licT]]-gfp, spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab [pubmed|23475962]
GP1229, [[gene|licT]]-yfp, spc), available in [SW|Jörg Stülke]'s lab [pubmed|23475962]
Labs working on this gene/protein
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
[SW|Josef Deutscher], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
The protein
additional information
information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000053 PRODORIC2 database]
Biological materials
Expression vectors
for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP165, available in [SW|Jörg Stülke]'s lab
for expression, purification of RNA-binding domain in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP315, available in [SW|Jörg Stülke]'s lab
for expression, purification of RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP572, available in [SW|Jörg Stülke]'s lab
labs
[SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
[SW|Josef Deutscher], Microbiology and Molecular Genetics, INRA Paris-Grignon, France